The aim of this study was to develop an aquaculture effluent treatment method employing eco-enzymes derived from the stems of banana trees. In this study, the levels of ammonia, nitrate, and nitrite in aquaculture effluents were measured using the Hach DR900 colorimeter. The results showed that the addition of 3 mL, 6 mL, and 9 mL of banana stem eco-enzymes reduced ammonia levels by 69.60%, 87.38%, and 93.20%, respectively.