Wound dressings are described as protective barriers that shield damaged epidermal tissues from the environment and expedite the curative procedure. The incorporation of antimicrobial agents in wound bandages arose from the recognition that bacterial infections were the primary impediment to wound healing. This research aims to comprehensively investigate the mechanical and antimicrobial characteristics of electrospun nanofibers infused with green-synthesized silver nanoparticles, crafted from chitosan. The objective is to deepen our understanding of the interplay between chitosan and silver nanoparticles and their potential in accelerating wound healing. The results indicated that nanofiber membranes composed of CT + PEO + AgNPs exhibited the highest tensile strength, rendering them suitable for practical wound healing applications. Additionally, these CT + PEO + AgNPs nanofibers were found to effectively inhibit bacterial growth, particularly showing enhanced efficacy against S. aureus compared to other bacterial strains. Furthermore, these nanofibers demonstrated antifungal activity against a range of fungi, with notable effectiveness against P. funiculosum but relatively lower efficacy against A. fumigatus and A. niger. The potent antibacterial activity of silver nanoparticles in preventing wound infections was underscored by microbiological analysis. This study underscores the potential of these nanofibers to support the body's natural healing processes and mitigate the risk of wound infections. Due to their combination of mechanical strength and antibacterial properties, these materials present a promising option for improving wound healing outcomes