Statement of problem: The process of selecting anterior teeth is crucial in complete denture treatment, impacting not just aesthetics but also functions like chewing and speech clarity. However, traditional methods for selecting these teeth lack a strong foundation and are based on facial measurements and proportions, which can differ significantly among various ethnic groups. Purpose: The purpose of the study wasto determine the correlation between various clinical landmarks and canine position and evaluating their efficacy in determining the position of the canine tooth. Material and Methods: Twenty subjects (10 males and 10 females) between the ages of 18 and 25, all with a complete set of six maxillary anterior teeth, participated in this study. Three reference lines—ala line (A line), inner canthus to ala line (IA line), and pupil line (P line)—were established. Horizontal distances from every reference line to both the canine cusp tip and distal contact point were measured and assessed using paired sample t-tests and one-sample t-tests. Results: In males, both the A line and IA line were observed to be closer to the canine tip compared to the P line. Conversely, among females, although not statistically significant (P > 0.05), the IA line exhibited closer proximity to the canine tip compared to both the A line and the P line. Significant differences were noted between all three reference lines and the canine distal contact point on both sides for both genders (p< 0.05). Conclusion: The IA line can effectively determine the location of canines in complete denture patients of both genders, whereas the A line is specifically reliable for determining the location of canines in males only. Clinical implication: The significance of facial landmarks in achieving a harmonious facial appearance cannot be overstated in the construction of complete dentures. When employing the A line method and IA line method in males, it's recommended that the line should align with the canine cusp tip. Conversely, in females, the IA line should align with the canine cusp tip. This gender-specific adjustment ensures accurate positioning of the lines relative to the canine teeth, optimizing the precision and aesthetic of dental procedures.