A wide variety of bioactive substances, such as alkaloids, phenolics, terpenoids, and flavonoids, are present in resin and are thought to be involved in its antibacterial qualities. Due to its therapeutic qualities, the resin of Artocarpus heterophyllus, better known as jackfruit, has long been employed in a variety of traditional remedies. The resin's possible antibacterial action is one of its noteworthy features. In this work, we examine the antibacterial activity of A. heterophyllus resin against a range of microorganisms and consider possible uses for it in the treatment of microbial illnesses. After gathering the resin from the bark of the plant, 200 mL of 100% ethanol were used to extract it overnight. The crude extract yield was gathered after the extract was filtered via Whatman No. 1 filter paper. The study's findings indicate that the resin extract exhibited a 100% concentration-dependent inhibitory zone against MRSA, S. aureus, E. faecalis, and S. mutans, but no inhibition against P. aeruginosa. At concentrations of 25% and 50%, there was very little inhibition. The phytochemical content of the resin, which includes substances like flavonoids, alkaloids, and tannins, may be responsible for its efficacy. The resin is of interest for possible pharmacological and medical uses since these bioactive compounds are known to hinder the growth and proliferation of bacteria.