ISSN : 2663-2187

Assessment of Phytochemical, Antimicrobial, Antioxidant activities and HPLC analysis of stem, leaves and fruits of plant Pyrus pashia

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Kanupriya Kuniyal , Niki Nautiyal , Geeta Bhandari
ยป doi: 10.48047/AF5BS.6.7.2024.1884-1898


People have long understood eating wild edible fruits has nutritional and therapeutic benefits. One of these lesser-known gems is Pyrus pashia, the Himalayan Pear or Wild Edible. This tree, which can grow up to 2,000 meters in elevation in the Western Himalayas and other temperate zones, is largely unutilized. Its many medicinal benefits have long been recognized by traditional medicine, which uses it to treat a wide range of conditions including eye infections, sore throats, diarrhea, abdomen problems, and many infectious diseases. The present work investigates the phytochemical screening, antibacterial, antifungal properties and antioxidant potential of different plant part extracts of Pyrus pashia and HPLC analysis of Ethyl acetate and Ethanol extracts for the analysis of three phenolic acids and three flavonoids. The stem, leaves, and fruits underwent extraction using various polar solvents such as water, ethanol, petroleum ether, chloroform, and ethyl acetate. Phytochemical screening revealed the presence of alkaloids, phenols, tannins, flavonoids, saponins, glycosides, and other compounds. The antimicrobial activity against multiple pathogens, including Enterobacter aerogenes, Klebsiella pneumoniae, E. coli, Penicillium sp., Aspergillus sp., and Rhizopus sp., was evaluated using the agar well diffusion technique. Furthermore, the antioxidant capacity of each extract was determined by assessing its ability to scavenge free radicals (2,2- diphenyl picrylhydrazyl). Pyrus pashiaexhibited great antimicrobial and antioxidant potential. The maximum property was seen in the extract of Ethyl acetate. HPLC analysis showed the availability of compounds of phenols.

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