Pistacia lentiscus L. is a shrub from the genus Pistacia, one of the Anacardiaceae families consisting of more than eleven species, widely distributed in the Mediterranean basin.This work was undertaken to enhance the different extracts studies of Pistacia lentiscus as a source of polyphenols and natural antioxidants, as well as the FTIR spectroscopy analysis, which was performed to identify the different function of this species. The screening of Pistacia lentiscus revealed the existence of bioactive compounds: flavonoids, phenols, tannins, anthocyanins and sterols. Pistacia lentiscus demonstrated a strong antioxidant activity for both extracts, with the highest values of total phenolics (4475.2 ±0.56 1.06 mg GAE/100 g) and flavonoids (29.52 ± 0.25 mg QE/100 g) found in the plant methanol extracts.The methanol extracts of the plants revealed highest values of total phenolics (4475.2 ±0.56 1.06 mg GAE/100 g), flavonoids (29.52 ± 0.25 mg QE/100g ), the evaluation of antioxidant activities showed a strong antioxidant activity of Pistacia lentiscus for the two extracts.