The utilization of plant-derived compounds has emerged as a promising source of bioactive substances with potential antimicrobial properties. Evaluating the antibacterial activity of fruit peel extracts from Citrus limon and Citrus limetta holds significant promise in exploring their applications against bacterial infections. Despite the common practice of discarding Citrus limon peel, it contains numerous bioactive compounds, as does Citrus limetta peel. Investigating the antibacterial potential of these extracts, if proven effective, could lead to their utilization as natural antimicrobial agents in pharmaceuticals, food preservation, or as substitutes for synthetic chemicals across various domains. This study aims to assess the antibacterial efficacy of fruit peel extracts from Citrus limon and Citrus limetta against various bacterial strains, thereby determining their potential as natural antimicrobial agents. Citrus limon and Citrus limetta peels were ground using a mortar and pestle and then extracted with ethanol. The resultant extract was further diluted to create different concentrations. Antimicrobial assay was (Zone of inhibition) conducted using Staphylococcus aureus (SA), Enterococcus faecalis (EF), Methicillin-resistance Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, and Streptococcus mutans as test organisms. The ethanol extract of C. limon and C. limetta peel extract was found inhibiting all the microbes at a maximum concentration of 100%. Among five different microbial pathogens, the extract the greater inhibition was found against S. mutans. In conclusion, the evaluation of antibacterial activity in fruit peel extracts from Citrus limon and Citrus limetta underscores their potential as natural antibacterial agents. Further investigations are warranted to isolate and identify the specific bioactive compounds responsible for this observed antibacterial activity.