Nosocomial infections are a significant cause of morbidity and mortality and are associated with costs and resource economization. The transmission of organisms through the hands of HCWs is a commonly known phenomenon. Changing gloves frequently is not practical and a frequently flouted procedure during the hospital rounds. Using gloves is not a substitute for hand hygiene. The aim of this study is to assess the efficacy of hand sanitizers to disinfect gloves between patients and to assess if this method has potential for further elaborate studies. Methods: 100 impression cultures and 300 swabs were collected from HCWs including Doctors and Nurses for culture and sensitivity attending OPD patients. Impression prints of the gloved hands were collected. Three swabs from each glove were obtained the study. Results: On sampling, 66% of the gloves used by the doctor’s showed presence of organisms, compared to only 52% donned by the nurses. And no growth was seen after using hand rub over the gloves. Conclusion: The principal enquiry was to know, how effective alcoholic hand rub in disinfecting gloves was after clinical examination. Using hand rub over the gloves is an effective way to disinfect and to prevent cross infections between the patients. This approach is user-friendly, reliable and safe.