Pyogenic infections are characterized by local and systemic inflammation usually with pus formation. (1) A break in the skin can provide entry to the surface bacteria which there to start multiplying locally. The body’s defense system produces immune cells in the area to fight against bacteria. In due course, the accumulation of these cells produces pus which is a thick whitish liquid. (2) Aims and objectives: 1) To determine the microbiological profile of aerobic organisms in pus and wound infection. 2)To determine the antibiotic sensitivity and resistant pattern of the microorganisms. Method: Culture has been grown from different specimen and antibiotic susceptibility testing done by Kirby bauer disk diffusion method as standard protocol in Microbiology Laboratory. Antibiotic resistance leads to prolonged hospital stays and increased costs in terms of treatment and causes life-threatening infections. The majority of MRSA strains worldwide have become resistant to multiple antibiotics including beta-lactams; tetracyclines; macrolides and more recently fluoroquinolones.(3) Reducing inappropriate antibiotic use is the best way to control resistance.(4). The microbiology laboratory plays a key role in the decision to choose a particular antimicrobial agent. Definitive treatment depends upon the appropriate selection of antibiotics according to the causative agent likely to work on the patient can be done. (5) Result : Out of 118 pus samples obtained in the microbiology laboratory from various departments of simmer medical hospital for aerobic culture and sensitivity, 77 ( 66%)samples yielded a positive culture whereas 40 (34%) samples yielded no growth .78 culture positive cases in 28 MDR cases organism isolates were found. Conclusion: All the isolates were subjected to antimicrobial susceptibility tests. Gram-positive isolates were most susceptible to vancomycin, levofloxacin and teicoplanin. Most gram-negative isolates were sensitive to imipenem, gentamycin, amikacin, levofloxacin, cefoperazone and cefepime.