Introduction The Urinary Tract Infections (UTIs) leading bacterial infection, mostly occur in all the community and hospital setups irrespective with their age and gender . At least half of women get experiences of urinary tracts infection for once in the life , due to anatomical structure of female urogenital system. Material and Method This study was conducted in the central laboratory of the Department of Microbiology, Dhiraj Hospital, Vadodara (Gujarat) between from January 2024 to June 2024. The study evaluated bacteriological profile and antibiotics sensitivity pattern of 175 clinically suspected of Urinary Tract Infections (UTIs), All 175 patients with clinical manifestation of UTI instructed to collect midstream potion of their urine by the clean ca tch technique in sterile wide mouth screw capped container which were distributed to them. Result This study was carried out at central laboratory of the Department of Microbiology, Dhiraj Hospital, Vadodara (Gujarat), between from January 2024 to June 2024 . Total 175 clinical manifestation of Urinary Tract Infections (UTIs) patient’s urine samples were. Out of these, only 54(30.85%) samples found positive microorganism growth. During analysis 97 and 78 samples were from female gender and male gender respect ively, out of this 36 (66.66%) and 18 (33.33%) cases were found from female and male gender respectively. This study shows that maximum cases of Urinary Tract Infections (UTIs) were found in 21 40 age groups which were 26. Conclusion The study reveals Uri nary Tract Infections (UTIs) is much more common in female gender due to their differing anatomy. E. coli is the predominant isolated pathogen in Urinary Tract Infections (UTIs) patients and study shows that bacterial which were isolated are resistant to t he routinely used antimicrobial drugs.