Introduction Pyogenic infections refer to infection causes pus formation. They are characterized by several local inflammations, usually multiplication of microorganism. Pus is a collection of thick, white or yellow fluid, formed at the site of inflammation during infection. It is made up of dead tissue, white blood cells, and damaged cells. Antibiotic r esistance among bacteria is becoming more and more serious problem throughout the world. That evolution of bacteria towards resistance to antimicrobial drugs, including multidrug resistance, is unavoidable because it represents a particular aspect of the g eneral evolution of bacteria that is un stoppable. Antibiotic resistance emerges commonly when patients are treated with empiric antimicrobial drugs. Monitoring of resistance patterns in the hospital is needed to overcome these difficulties and to improve the outcome of serious infections in hospital settings. Materials and Methods A retrospective study was conducted at Dhiraj hospital, SBKS MI & RC from January to May 2024. All pus samples collected during the study period were included. Pus samples were aseptically collected using sterile swab in a test tube and inoculated on to blood agar and MacConkey agar. Plates were incubated at 37◦C for 24 hours. Organisms were identified by series of biochemical reactions standard following standard procedures. Ant imicrobial susceptibility testing was performed using Muller Hinton agar plates by disc diffusion method following Clinical and Laboratory Standards Institute (CLSI) guidelines. Results Of 125 pus samples collected, 37(29.6%) were positive cultures, which included 16 (43.2%) Gram negative and 21(56.7%) Gram positive bacteria. and no growth in 88(70.4%) samples. Middle age group of 20 40 years 48 (38.4%) was most commonly affected age group. Males 68(54.4%) were commonly affected than females 57(45.6%). Con clusion The spread of the beta lactamase producing organisms has been increasing. The Present study showed increased resistance to beta lactam antibiotics which is serious problem.