The incorporation of biogenic silver nanoparticles (AgNPs) into a film matrix of polyvinyl alcohol (PVA) and polyethylene glycol (PEG) offers a promising opportunity for diverse biomedical applications. In this research, we successfully synthesized biogenic AgNPs using plant extracts through an environmentally friendly synthesis method, and then integrated them into a PVA/PEG film. The resulting film was thoroughly assessed for its antibacterial, antioxidant, and cytotoxic effects on MCF-7 human breast cancer cells.The biogenic AgNPs synthesized were analyzed using various techniques including UV-Visible spectroscopy, scanning electron microscope (SEM) and Fourier-transform infrared spectroscopy (FTIR). The PVA/PEG film incorporated with AgNPs exhibited significantly improved antibacterial activity against both Gram-positive and Gram-negative bacteria compared to the pristine PVA/PEG film. This was demonstrated through agar diffusion assay.Furthermore, the antioxidant activity of the film was evaluated using DPPH radical scavenging assay, demonstrating notable radical scavenging potential.This multifunctional film has great promise for a variety of biological applications, including wound dressings, implant coatings, and anticancer treatments. More study is needed to better understand its mechanisms of action and optimise its efficacy for therapeutic use.