The present investigation entitled “Biochemical screening of sawan millet (Echinochloa frumentacea) grown in Devipatan Mandal of Eastern U.P.” was carried out in the laboratory of Department of Biochemistry, N.D. University of Agriculture and Technology, Kumarganj, Ayodhya. Ten germplasm of sawan millet were selected as the experimental material. NDS-1, NDS-2, NDS-3, NDS-4, NDS-5, NDS-6, NDS-7, NDS-8, NDS-9, NDS-10, were collected from Devipatan mandal, and used as experimental materials in the field trail. The various biochemical parameters such as protein content NDS-5 (9.33 per cent), crude fibre content NDS-9 (6.90 percent), Total mineral content NDS-5 (2.29 percent), tryptophan content NDS-2 (39.33mg/g), lysine content NDS-10 (85.33mg/g), methionine content NDS-1 (107.33mg/g), were also noticed in present investigation. Antinutritional factors such as Phytic acid content NDS-2 (85.33mg/100g) and Tannin content NDS-6 (59.85mg/100g) were found reported minimum in the present germplasm, so the germplasm were utilized in the further research work.