This study investigates the potential of bioengineered silver nanoparticles (AgNPs) derived from Crateva religiosa G. Forst. bark as peptide inhibitors for targeting cancer cells. The synthesis of AgNPs was achieved through a green and eco-friendly approach, utilizing the aqueous extract of C. religiosa bark. The synthesized AgNPs were characterized using various analytical techniques to confirm their size, morphology, and surface properties. Peptides derived from the bark were identified and screened for their inhibitory effects on cancer cells. The anticancer activity of both AgNPs and peptide inhibitors was evaluated using cell viability assays, cellular uptake studies, and molecular docking simulations. Results demonstrate that the bioengineered AgNPs exhibit significant cytotoxic effects on cancer cells, with enhanced efficacy when conjugated with bark-derived peptides. Furthermore, molecular docking simulations reveal potential binding interactions between the peptides and key biomolecules involved in cancer progression. Overall, this study highlights the promising potential of bioengineered AgNPs derived from C. religiosa bark in cancer therapy, particularly when combined with bark-derived peptides as targeted inhibitors against cancer cells