With the revolution in the field of science, nanotechnology has evolved as one of the most promising areas which has good promising potential capabilities. But this fact cannot be neglected that nanotechnology has not expanded its full roots in the area of agriculture and the environment. As these nanoparticles have different structures and shapes such as nanospheres, nanocubes, and nanoshells, thus they are considered as one of the most stable materials. On the other hand, the advent of plant endophytes is also simultaneously emerging as one of the key sources to explore different interactions and plays a pivotal role in promoting plant growth and crop improvement. The association of endophytes with nanoparticles could provide a great breakthrough in the bioprospecting of a sustainable environment. Endophytes have the potential to produce various types of nanoparticles that have anti-pathogenic properties against many pathogenic microorganisms that are responsible for causing diseases in humans as well as in plants. The biological synthesis of nanoparticles that can be explored from endophytes can be efficient enough to control all forms of pathogens. This will in return contribute to the environment in a very effective manner. This paper will focus on exploring the importance of endophytic nanoparticles in the form of nano-fertilizers, nano-bionics, etc. also focussing on major breakthrough and its prospecting role in the environment.