Introduction: Steroids are significant biomolecules, such as cell membrane components and hormones, because of their biological activity. Steroid drugs show a wide range of biological significance, such as anti-inflammatory, immunosuppressant, contraceptive, and anticancer properties.There are reports of certain derivatives with anticancer or antileishmanial properties.Aim:Biotransformation of testosterone by fungus and the experimental methods: A Mini-Review.Methods: An extensive search was performed in different databases.Scopus, Embase, Cochrane Library, and PubMed.The keywords used in the search were fungal biotransformation, steroidal fungal biotransformation, and fungal biotransformation.All studies published in English and those on fungal biotransformation were included in the analysis.Results: Biological transformation of steroids is a better alternative to chemical synthesis because biocatalysts are more stereo- and regio-specific.The biotransformation of steroids is an important area of research in microbiology. Although bacteria, algae, and yeast can transform steroids, many fungi from diverse taxa transform steroids of various classes.The obtained products are important for pharmaceutical and therapeutic applications.Conclusion: This review summarises the fungal fermentation of androstane, elaborating on testosterone biotransformation, which takes place in 17 different genera, and the experimental methods followed by various researchers in the extraction, analysis, and structural identification of products.