Diabetes, a persistent degenerative metabolic condition associated with significant morbidity and mortality rates due to its complications, has prompted increased exploration into novel bioactive compounds for its treatment. This quest includes the investigation of metabolites derived from marine sources. Bryostatin 1 a cyclic macrolide originally isolated from a marine organism Bugula neritina. In recent years, bryostatins have garnered significant interest due to their diverse range of biological activities. It is a macro lyric lactone that activates protein kinase C and modulate cell signaling pathways. In light of the given background, the present study has aimed to investigate the anti-diabetic efficacy of Bryostatin 1 and its potential for the management of diabetes associated complications, MATERIALS AND METHODS: The anti-diabetic efficacy of Bryostatin 1 was evaluated in in vitro conditions using -amylase and -glucosidase, carbohydrate hydrolyzing enzymes. Further, the mechanism of action was evaluated by Dipeptidyl peptidase-4 (DPP-IV) inhibitory activity. The effect of Bryostatin 1 on diabetes associated complications was assessed by evaluating its effect on advanced glycation end products (AGEs) and sorbitol accumulation. RESULT: The results of the present study showed significant inhibition of carbohydrate hydrolyzing enzymes, -amylase and -glucosidase by Bryostatin 1 at varying concentrations which is compared with that of Standard acarbose. The DPP-IV activity was found to be markedly inhibited in a dose-dependent manner. The effect of Bryostatin 1 on AGEs formation and aldolase reductase was found to be significantly attenuated indicating its potential role in the management of diabetes associated complications viz., nephropathy and retinopathy. CONCLUSION: The current study results sheds light on the potential of Bryostatin 1 as a novel therapeutic agent for diabetes mellitus and its associated complications. Nevertheless, additional in vivo investigations are necessary to confirm the safety and effectiveness of the compound, with a more comprehensive focus on uncovering its mechanism of action