This paper investigates the use of nanoparticles composed of Chitosan and Terminalia arjuna extracts for protecting cardiac cells from lead acetate induced damage in rats. The Chitosan Terminalia arjuna extract nanoparticle was analyzed using Dynamic Light Scattering (DLS) and scanning electron microscopy (SEM). The study involved three groups of 100 rats each: one group received only purified water, another group received intraperitoneal injections of l ead acetate (15 mg/kg BW), and a third group received either lead acetate injections or oral doses of Chitosan Terminalia arjuna nanoparticles at 100, 200, or 400 mg/kg BW. Blood samples were collected to measure concentrations of creatinine kinase MB (CK MB) and lactate dehydrogenase (LDH). Additionally, levels of glutathione peroxidase (GPx), superoxide dismutase (SOD), malondialdehyde (MDA), and heart damage were evaluated histologically in cardiac tissues. SEM analysis revealed that the Chitosan Termina lia arjuna extract nanoparticles had a rough surface and irregular shape, with a size of 312.9 ± 25.7 nm as determined by DLS. Lead acetate exposure resulted in increased levels of LDH, CK MB, and MDA, while it decreased SOD and GPx levels. Histological as sessments confirmed the lead acetate induced damage to cardiac cells. Regardless, at 400 mg/kg BW, a nanoparticle comprising Terminalia arjuna extract and Chitosan reduced LDH, CK MB, and MDA levels while boosting SOD and GPx. Plus, the nanoparticle with 4 00 mg/kg BW of chitosan and Terminalia arjuna extract damaged cardiac cells more extensively overall. The findings provide credence to the theory that the Chitosan Terminalia arjuna nanoparticle shields rats from lead acetate induced cardiotoxicity by virtue of its potent antioxidant characteristics.