In today’s industrial revolution, many industries are generating different types of waste impurities which are harmful & toxic for environment as well as for human life. Many researchers had showed interest in overcoming these by using various types of biosorbents with adsorption process to extract the hazardous metals from the industry wastewater. Below paper mainly focuses on three biosorbents namely Neem leaves powder (NLP), Tea wastes (TW) & Sugarcane bagasse (SCB) with their preparation, composition & analysis for use in accomplishing adsorption process to be more effective. Current paper reveals the numerous functional groups responsible for adsorption process & to be detected on exterior surface of biosorbents i.e. Neem Leaves Powder, Tea wastes & Sugarcane bagasse by performing FTIR analysis. It also depicts biosorbents surface morphology at different magnifications by performing SEM analysis. Also, describes Physico-chemical analysis of all biosorbents taken into consideration for analyzing the effectiveness as well as suitability as a biosorbents in doing adsorption to be more conducive.