Plant pathogenic bacteria cause severe damage to crop yields and product quality in Vietnam, as well as in many countries around the world. The objective of this study was to isolate and identify new sources of biological materials that exhibit resistance to plant pathogens. In this study, a total of 72 different strains of actinomycetes were isolated from soil samples collected from various locations. Among these strains, CP1.3 demonstrated intense antagonism towards five tested bacteria and the Fusarium oxysporum fungus. The CP1.3 strain displayed robust growth and exhibited strong antibacterial activity at a temperature of 30℃, pH range of 7.0-8.0, and salt concentrations up to 1.0%. It also utilized D-glucose, D-sorbitol, and maltose as good carbon sources while producing extracellular enzymes such as chitinase, cellulase, and protease. By analyzing the nucleotide sequence of CP1.3's 16S rRNA and compared to the nucleotide sequence from Genbank, it revealed that this strain has a close relationship with the Streptomyces aureofaciens species (99.03% similarity). As a result attained, the strain CP1.3 was named as Streptomyces aureofaciens CP1.3.