Background: Chronic dacryocystitis is an infection of the nasolacrimal sac caused due to obstruction of the nasolacrimal duct. Cases of chronic dacryocystitis if not treated on time, will lead to complications like conjunctivitis, corneal ulcer, lacrimal abscess, fistula or orbital cellulitis, endopthalmitis and hypopyon. Aim: To study the current pattern of antibiogram of aerobic bacterial isolates from cases of chronic dacryocystitis. Material and methods:This is a cross sectional study conducted over a period of one year by the Department of Microbiology and Ophthalmology at Jawaharlal Nehru Medical College, KAHER, Dr Prabhakar Kore Hospital, Belagavi. A total of 80 cases of chronic dacryocystitis which were clinically diagnosed at the ophthalmology OPD were included in this study. Discharge was collected using sterile cotton swab, ensuring not to touch the conjunctiva nor the eyelid margin. Specimens were then immediately sent to the Department of Microbiology for further process. Gram staining and inoculation on Blood agar and Macconkey agar was done followed by incubation of the plates at 37°C for 24 to 48 hours. Organisms were identified and antibiotic sensitivity testing by Kirby Bauer disc diffusion method was done according to the standard CLSI guidelines.