Background: Laparotomy for perforation is a common surgical intervention associated with a risk of postoperative wound infection. Preoperative intra-incisional antibiotic infiltration has been proposed as a preventive measure to reduce this risk. This study aimed to compare the rates of wound infection in patients undergoing laparotomy for perforation who received preoperative intra-incisional antibiotic infiltration versus those who did not. Methods: A prospective comparative analysis was conducted on 100 patients who underwent laparotomy for perforation at myhindore between june 2018 to may 2019.Patients were divided into two groups based on whether they received preoperative intra-incisional antibiotic infiltration or not. Wound infection rates were compared between the two groups using appropriate statistical methods. Results:We looked at wound healing three days after surgery in two groups using a chi-square test. The result, with a chi-square statistic of 4.6095 and a p-value of 0.329758, indicates that the difference between the groups isn't statistically significant. We analyzed wound status seven days after surgery in two groups using a chi-square test. The result, with a chi-square statistic of 36.4104 and a p-value less than 0.00001, shows a highly significant difference between the groups. Conclusion: Our study findings suggest that preoperative intra-incisional antibiotic infiltration may have a potential role in reducing wound infection rates in patients undergoing laparotomy for perforation. Further prospective studies are warranted to validate these findings and determine the optimal strategies for infection prevention in this patient population.