This study evaluates and compares the effectiveness of two diagnostic methods for detecting Hepatitis B surface antigen (HBsAg) are EnzymeLinked Immunosorbent Assay (ELISA) and the iminochromatographic test. ELISA, a widely utilized laboratory technique, offers high sensitivity and specificity but necessitates specialized equipment and technical expertise. In contrast, the iminochromatographic test is a rapid method that provides quick, user-friendly results, though its accuracy can vary. Evaluation, sensitivity, specificity of two methods were assesedusing a patient cohort. Our findings indicate that while ELISA remains the gold standard for HBsAg detection, the iminochromatographic test is a reliable alternative in resource-limited settings, albeit with slightly lower sensitivity. This comparative analysis underscores the importance of selecting diagnostic tools based on context, balancing accuracy with accessibility.