Fishes are the most important source of food in all over the world. They are also easily infested by endo-parasites and ecto-parasites in both fresh and sea water ecosystem. The present study was conducted to estimate the infestation index of helminth parasite in Channa punctata, Anabus testudineus and Oreochromis mossambicus from Vasai- Virar region of Palghar district during July 2021 to December 2022. The study helps to predict the comparative and present status of fresh-water bodies of Vasai region i.e Chikaldongri and Satpala lake. It was found that the infestation index of helminth parasite in Oreochromis mossambicus and Channa punctata is less than Anabus testudineus. During summer season maximum parasite was collected from freshwater fishes. Also, it was observed that due to drastic anthropogenic activities held in study area since 2017, the fish species have started to decline because of loss of habitat and vigorous pollution with illegal constructions and activities. The analysis of data shows the occurrence of helminth parasites vary according to the season in freshwater fishes. Helminth parasite and host species, host size, feeding habitats and seasons and localities also influence the intensity. After data analysis, the study was concluded that the high infections of helminth parasite were occurred in summer followed by winter and low in rainy season. Thus, studies postulated that the environmental factors and feeding habitat are influencing the seasonality of parasitic infestation directly or indirectly. The present work also indicates that the helminth (Nematode) parasite is extensively distributed in stomach and digestive system of edible freshwater fish Channa punctatus. It is postulated that, “due to the occurrence of these parasites, the physiological activities of the victimized fishes are hindered. Parasites may lead to epidemics and mortalities, resulting in economic loss in a particular area. Besides this, there is always the possibility of their transfer to the human beings by consumption of raw and partially cooked infected fishes.” Post- pandemic studies revealed low availability of fish species in study area