Guduchi, commonly known as Tinospora cordifolia or Giloy, is a highly esteemed medicinal plant in traditional Indian medicine, known for its many therapeutic properties. Although it is frequently exploited, detailed comparative studies of the male and female plants of this species are lacking. This study aims to reveal the differences between male and female T. cordifolia plants by a comprehensive physiochemical analysis. Also, a comparative study between the Kwatha prepared by both samples were analyzed. Physicochemical analysis revealed that while both genders contain similar types of bioactive compounds, there are notable quantitative differences between them. Both male and female T. cordifolia plants exhibit nearly identical results, showing minimal discernible differences like pH values, alcohol soluble extractive values and ash values in which female variety showing slightly better values than male plant of Guduchi. These findings emphasize the need to consider plant gender when using T. cordifolia medicinally, suggesting that targeting the use of male or female plants could improve therapeutic effects for specific health conditions