ISSN : 2663-2187

Comparison of Corn meal broth method and Dalmau plate method for demonstration of Chlamydospores in Candida albicans

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Dr Natasha v, Dr Nawaz Umar, Dr Trupti V, Dr Sangeeta K, Mr Mangal Murthi G
» doi: 10.33472/AFJBS.6.6.2024.5629-5632

Abstract

Candida occurs as normal commensal in healthy individuals, and is usually present on the skin and mucosal surfaces of the oral cavity, gastrointestinal and vagina and urogenital tracts .However it can cause a wide variety of infections in immunosuppressed patients like those on broad – spectrum antibiotics, steroids, immunosuppressive drugs, diabetes mellitus, AIDS, Malignancy, Neutropenia. Majority of infections caused by Candida albicans .Non- C. albicans Candida (NCAC) species are emerging as important pathogen in humans ..Our study included 44 clinical isolates which showed gram positive budding yeasts cells with or without pseudohyphae and Germ tube positive. In this study we compared liquid media and slide culture for the rapid production of chlamydospores. Among 44 C.albicans, with broth method 38 (86.6%) showed single terminal chlamydospores at 16 hours of incubation. Standard dalmau plate method showed budding yeast cells with pseudohyphae without chlamydospores at 16 hours but after 72 hours all the isolates showed characteristic chlamydospores.

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