Objective: This study investigated the potential toxicity of coumarin in cinnamon powder and cinnamon formulations. The focus was on the extraction, separation, and identification of coumarin and other essential oil components. Method: Components from cinnamon samples were extracted using various solvents based on their polarities. Thin layer chromatography (TLC) was employed to screen the compounds. Essential oil components, including coumarin, were separated using column chromatography. Result: The extraction process successfully isolated essential oils and water-soluble components from the cinnamon samples. TLC screening confirmed the presence of key compounds such as cinnamaldehyde, eugenol, and coumarin. Column chromatography effectively separated these compounds, allowing for the detailed analysis of their presence and concentration in the samples. Conclusion: The study confirmed the presence of coumarin in cinnamon powder and formulations. Given the known toxicity of coumarin in animals and its potential risks to humans, these findings underscore the importance of monitoring and regulating coumarin levels in cinnamon-based products. Further research is recommended to fully understand coumarin's effects on human health and to develop guidelines for safe consumption.