Background and Aim: Abrasion on the crown is commonly seen with usage of toothpastes since many of them contain active abrasive components. The exclusive aim of this in-vivo study was to assess the abrasive potential of three commonly utilized pediatric toothpastes on new millennium crowns used in pediatric dentistry. Materials & Methods: Both male and female pediatric patients were included in this study in the range of 9-12 years. Total thirty teeth were selected for study in which New Millennium Crowns were placed. Three commonly used popular toothpastes were chosen for study. First study group (Group 1) included 10 patients in which Kidodent Bubble Fruit Flavour Kids Toothpaste were used. Group 2 included 10 patients in which Baby Organo Herbal Kids Toothpaste Strawberry was used. Group 3 included 10 patients in which Himalaya Kids Bubble Gum Toothpaste was used. The extent of abrasion was measured by Non Contacting Laser Profilometry. P value less than 0.05 was considered significant (p< 0.05). Statistical Analysis and Results: In this study, all 3 study groups have 10 teeth each. After 2 months, p value was highly significant in group III (0.020). Mean abrasion (measured in micron) was 1.41, 1.98 and 2.21 respectively for group I, II, III. After 4 months, p value was highly significant in group III (0.010). Mean abrasion (measured in micron) was 1.69, 2.12 and 2.31 respectively for group I, II, III. One-Way ANOVA analysis exhibited highly significant p value (0.002). Estimations of Two Sample T- Test also exhibited highly significant p value (0.001). Conclusion: It was concluded that abrasion was maximum on the New Millennium Crowns subjected to Himalaya Kids Bubble Gum Toothpaste. Likewise, abrasion was found minimum on the New Millennium Crowns subjected to Kidodent Bubble Fruit Flavor Kids Toothpaste. This pattern was recognized in the estimations made at 2 months and 4 months timings among all three tested toothpastes.