Lichens, complex symbiotic entities composed of fungi and algae, produce unique secondary metabolites with notable bioactive properties. This study aimed to collect, classify, and conduct preliminary phytochemical analysis on five lichen species: Stereocaulonfoliolosum, Everniastrumcirrhatum, Usnea longissima, Lobariaretigera, and Peltigerapolydactylon. Specimens were sourced from the alpine zones of Rudraprayag and Uttarkashi districts in Uttarakhand. To determine the optimal solvent for secondary metabolite profiling, we used petroleum ether, acetone, and methanol. Methanol was identified as the most effective solvent for phytochemical assessment. Specimens were preserved in the LWG herbarium at the National Botanical Research Institute (NBRI), Lucknow, for future reference. The uses and significance of these lichen species were documented through literature review and interactions with indigenous residents of the Garhwal region in Uttarakhand.