Vitamin deficiency is the lack of essential vitamins in our body and turns out to be a matter of major concern when it continues for the long term causing various abnormal signs and symptoms. Vitamin D is a group of fat-soluble prohormones known as sunshine vitamin. The two major biologically inert precursors of vitamin D are vitamin D3 (cholecalciferol) and vitamin D2 (ergocalciferol). Vitamin D plays an important role in maintaining an adequate level of serum calcium and phosphorus. Vitamin D has a great effect on forming and maintaining strong bones. Vitamin D deficiency occurs when people do not have an appropriate dietary intake or exposure to UVB rays. Ayurveda emphasizes prevention and encourages health maintenance through close attention to balance in one’s life, right thinking, diet, lifestyle, and the use of medicines. Knowledge of Ayurveda enables one to understand how to balance body, mind, and consciousness to lead a healthy life. In the present era food habits, life lifestyle of humans has changed a lot. Even there is a drastic change in environment and climate. Hence, newly formed diseases not explained in the Ayurveda Samhita are emerging. Here an attempt was made to understand the Vitamin D3 deficiency with the basic principles of Ayurveda. This study would help a further researcher in establishing the principles and treatment for this condition. Current medical science can discover only a few facts about the mechanism of vitamin D3 Deficiency but fails to understand its complete etiology. With the above facts in mind, can a scientifically designed, well-planned study gives some answers to this ever-increasing health hazard, this was the motto of working on this problem.