Helicobacter pylori (H. pylori) is a gram-negative microaerophilic bacterium found in the mucous linings of the stomach, representing a prevalent and significant transmissible human bacterial pathogen. Timely diagnosis of infection is crucial for effective treatment and to mitigate potential complications. The gold standard for detection involves morphological assessment of gastric biopsies for H. pylori. This study aims to evaluate two cytological methods (Brush cytology and Touch imprint cytology) and compare their efficacy against the gold standard method. This study examines the efficacy of two methods for detecting H. pylori in gastric biopsies: endoscopy brush sampling and touch imprint cytology. Biopsies were obtained from dyspeptic patients undergoing routine upper gastrointestinal endoscopy. The specimens were analyzed using both methods and reviewed by three independent pathologists blinded to the cases. The cytological approach proves to be more efficient in promptly detecting H. pylori organisms compared to routine histopathology sections, exhibiting excellent sensitivity. Therefore, with adequate training and expertise, cytology can serve as a convenient bedside testing method for rapid and accurate H. pylori detection. This facilitates clinicians in promptly initiating targeted antimicrobial therapy.