The study questions are focused on the concept of a complex linkage between the microbiome composition of the gut and the HBV replication process dynamics. The mechanisms that might be impacted by gut microbiota on HBV infection outcomes are going to be explored through computation-based tools. These mechanisms, when understood thoroughly, form the basis of proposing new treatment strategies against chronic hepatitis B viral infection. Research work starts with a wide range of techniques consisting of bioinformatics, statistical modeling, and data visualizations. By showing the gut microbiome’s effect on HBV pathogenesis, the research paper instructs the truth. It also contributes to our increasing knowledge about viruses and how they affect the microbiota-host interaction. This may give a way for directly addressing the gut microbiome concerning altering HBV replication and variation in viral load to have more precise individual treatment of HBV.