From the insights gained by evaluating pyrogallol on both plants and animals, we have reported its toxicological effects in this research. The compound was exposed to zebrafish and the histopathological evaluations of gill and intestine were performed. Likewise, the impacts were assessed in the germinating Pisum sativum for a period of about 7 days. Finally, a 3% human RBC suspension incubated individually at pH 6, 7 and 8 were used to know pH-dependent hemolysis caused by pyrogallol. The gill parenchyma became dislodged and the lamellar epithelia were seen to be degenerated in the gills of zebrafish. In the intestine, inflammation was observed with accumulation of infiltrates in the mucosa layers. Further, Pisum sativum seeds showed stunted growth parameters during germination due to the application of pyrogallol. At acidic and neutral pH, destruction of human erythrocytes did not take place whereas, at alkaline condition (pH 8), hemolysis seemed to occur at a rate of about 13.2 ± 0.04%. Thus, these research findings suggest that pyrogallol could cause potential toxicity to many life forms damaging the environment and hence, the usage of this chemical compound must be limited.