INTRODUCTION: Methicillin-resistant Staphylococcus aureus (MRSA) is an important human pathogen associated with nosocomial and community-acquired infections.1 The mecA gene is considered to be one of the important virulence factors of S. aureus responsible for the acquisition of resistance to methicillin.2 The main aim of this study was to investigate the prevalence, pattern of antibiotic susceptibility and mecA gene.3 METHODS: A total of 110 MRSA isolates with mecA gene were isolated from 545 isolates. Antibiotic susceptibility testing (AST) was performed by the Kirby-Bauer disc diffusion method using cefoxitin and genotypic testing of the mecA gene by PCR. RESULTS: Of 545 isolates, 110MRSA isolates with mecA gene were detected and were multiple drug resistant (MDR). In AST, gentamicin and amikacin were found to be the least effective, while vancomycin was the most effective. The prevalence of the mecA gene was 20.18% (110/545). CONCLUSION: This study concludes that PCR is highly sensitive for the detection of the mecA gene.