Objective: An investigation was carried out to assess the total phenolic, antioxidant characteristics, antioxidant components, and antioxidant enzyme activity in developed cherry tomato breeding lines at the point of harvest when the tomatoes are completely mature and have turned red in colour. Methods: The tomato samples were successfully identified by the Department of Life Sciences at Manipur University (BOTANY) as Solanum Peruvianum (wild cherry tomatoes). Phenolic compound determination was carried out using Folin ciocalteu reagent (McDonald et al. 2001). DPPH Activity, Total Antioxidant Assay-FRAP and ABTS Assay was performed. Results: The phenolic content was estimated in sample Tomato (111.9394 μg GAE/mg) i.e. 1 mg sample was found equivalent to 111.93 μg of the Gallic Acid. 50% inhibitory concentration and the antioxidant activity (DPPH Assay) of the tomato sample were determined based on the experimental study. The sample's ability to scavenge DPPH was discovered. Results showed that the tomato sample had imitated antioxidant activity (FRAP) and a 50% inhibitory concentration. Compared to ascorbic acid, the tomato sample had far reduced action. The standard ascorbic acid equalled 469.2 μg of the tomato sample. The ABTS Radical Scavenging Assay revealed that the tomato sample shown a marginal enhancement in activity when compared to standard ascorbic acid, as evidenced by a 50% inhibitory concentration. The results showed that 78.47 μg of tomato sample was equivalent to 2.311 μg of conventional ascorbic acid. Conclusion: The study analysed cherry tomato breeding lines' antioxidant content, features, components, and enzyme activity, revealing significant antioxidant activity and bioactive ingredients, potentially contributing to health benefits.