Using a two-step melting process, the drug Acyclovir was put inside biodegradable gelatin nanoparticles. Glutaraldehyde was used as a cross-linking agent, and gelatin was used as a biodegradable material in this process. The goal was to aid the body in getting the medicine. The study used the Design Expert computer program to look at what happened when the amounts of gelatin polymer (A1) and the glutaraldehyde-crosslinking agent (A2) were changed. They looked at how particle size (B1), zeta potential (B2), and trapping efficiency (B3) changed. We looked at the drug-loaded gelatin nanoparticle mixtures by measuring how well they trapped the drug, how charged the surface was, and how big the particles were. ANOVA tests were used to look at the gelatin nanoparticles that had drugs in them. One part acyclovir to eight parts gelatin made up the improved version (F5). It had 0.8% gelatin polymer (A1) and 250 μL of glutaraldehyde (A2), a bonding agent. The best trapping rate for this mixture was 91.00%, the zeta potential was -29.88mv, and the particle size was 140.01 nm.