Cubosomes are square and rounded, discrete sub-micron, nanostructured particles with internal cubic lattice of the bicontinuous cubic liquid crystalline phases. These are thermodynamically stable. Drug and other substance penetration through skin are influenced by the physiochemical properties of the penetrant, the state of the skin, and the nature of the vehicle at relatively slow rates. Antiseptics, antifungals, anti-inflammatory medicines, and skin emollients for protective benefits are among the drugs used topically, primarily for local action. Actinic keratoses (AKs), also referred to as senile keratoses or solar keratoses, are benign intra-epithelial neoplasms which is often associated with chronic sun exposure, individuals with AKs may present with irregular, red, scaly papules or plaques on sun-exposed regions of the body. If left untreated, AKs may evolve into invasive squamous cell carcinoma, which underscores the importance of early detection and development of a treatment plan. Actinic keratosis (AK) is a chronic disease which is mainly located across areas of sun-exposed skin. In the present work, the cubosomes were prepared by utilizing GMO and polymer. The optimal formulation size could be obtained when 100 mg of glyceryl monoolein, 33.793 mg of polymer, and 10 mg of drug as independent variables leading to the formulation of cubosomes with 128.3 nm in mean diameter and 78.852% in encapsulation efficiency. Prolonged release was achieved when they were formulated as topical gels on maintaining the cubosome structure