Toxoplasma gondii was first isolated from a common gundi (Ctenodactylus gundi) in Tunis in 1908 and the same year in a rabbit from South America. Six clades have been characterized using population genetic structure studies indicating that globally diverse isolates originate from a small number of ancestral lineages .The diagnosis is critical for the surveillance, prevention and control of toxoplasmosis. Traditional approaches to the laboratorial diagnosis involve etiological, histopathological, immunological, immunohistochemical and molecular methodologies. Recently, two biological approaches are used: (i) direct detection as parasitological methods using microscopy to parasite detection and (ii) indirect detection through immunological assays. The other is the molecular methodology that detects the parasite DNA in suspected samples.