Suspected sepsis is one of the most frequently challenging diagnoses in neonates, the clinical signs of infection are not specific and late. Blood culture is the golden standard but time consuming in neonatal sepsis diagnosis provides late information. Aim of work: Evaluation of the Rodwell scoring system, Cluster of Differentiation 64 (CD 64), and Cluster of Differentiation 11b (CD11b) expression as markers in cases with culture proven sepsis. Patients and methods: 107 patients with culture proven sepsis were enrolled in the cases group and 118 patients were enrolled in the control group. Both groups were examined for monocytic and neutrophilic CD11b and CD64 expressions analyzed by flowcytometry. Complete blood count (CBC) was analyzed and Rodwell scores (hematological scoring system) were calculated for both groups. Results: CD64 on both monocytes and neutrophils was significantly higher among patients with culture proven sepsis compared to those in the control group with sensitivity of 94.9% and 76.2% (P< 0.001) and specificity of 59% and 83% (P< 0.001), respectively. Rodwell score was significantly higher in cases group compared to the control group with sensitivity of 87.85% and specificity of 94% (P<0.001). Conclusion: Rodwell scoring system is a simple screening test for early diagnosis of neonatal sepsis. It may help clinicians in identifying sepsis and to institute proper anti-biotic therapy