Introduction: Arterial stiffness is a critical predictor of cardiovascular events and mortality. Recent studies suggest that vitamin D may play a role in modulating arterial stiffness. Materials and Methods: We conducted a cross-sectional study among 96 age-matched individuals of both genders between the normotensives and hypertensives to explore the intricate relationship between arterial stiffness and total vitamin D level. SPSS software version 20 was used for analysis of data which was presented as mean ± standard deviation (SD) after ttest. Correlation coefficient (r-value) was derived between the parameters by using Pearson’s correlation while p<0.05 was considered statistically significant. Results: We found a significant (p <0.001) difference for Arterial Stiffness Index (ASI) of different limbs, and vitamin D between our study groups. Our results suggest a positive correlation (r = 0.67, p <0.001) between arterial stiffness and blood pressure. We also observed a negative correlation (r = -0.63, p <0.001) of arterial stiffness with serum total vitamin D levels and blood pressure and vitamin D (r = -0.87, p <0.001) levels respectively. Conclusions: We conclude that higher vitamin D levels are associated with lower arterial stiffness, and vitamin D supplementation may be beneficial in reducing arterial stiffness, particularly in individuals with low baseline vitamin D levels.