Introduction:A big worry nowadays is the rise in drug resistance to antifungal medications brought on by Candida species, which cancause a variety of clinical conditions, from mucocutaneous infections to invasive diseases that can be fatal. Biofilm production is the primary cause of most diseases caused by Candida spp. In order to effectively treat Candida infections, it is important to identify Candida, evaluate its susceptibility to antifungals, and determine its capacity to build biofilms. Aim and objective: To speciate Candida isolates from various clinical specimens and to detect biofilm formation by phenotypic methods and to do antifungal susceptibility. Methods:Total 70 candida isolates were isolated and speciated as per conventional methods. Antifungal susceptibility testing was performed using disc diffusion testing in compliance with CLSI guidelines. Three phenotypic methods—the Tube technique (TM), the Microtitre plate method (MTP), and the Congo Red Agar (CRA) method—are used to detect biofilm.