To develop the Bio chemical variables among university hockey players to practice the circuit training and ladder training. Purpose: This study persevered in determining whether the circuit and ladder training effect physical variables such as Low-Density Lipoprotein (LDL) and High-Density Lipoprotein (HDL) among university level Hockey players. For this study 60 hockey players were randomly chosen from Arts & Science Colleges affiliated to Alagappa University were selected as subjects and their age range between 18 to 25 years. Methods: There were four groups of 15 players each, total subject were 60 University hockey players. The group A Circuit training is followed by group B ladder training is followed by group C Combined training and the control group D have not Underwent any training and the data was before and after twelve weeks of training. A pre-test was administered two days before the training period began, and a post-test was administered after the training period concluded. The following variables namely Low-Density Lipoprotein and High-Density Lipoprotein were selected as criterion variables. All the subjects of four groups were tested on selected dependent variables at prior to and immediately after the training programme by using standardize testing tool respectively. Conclusion: Combined Circuit training, ladder training has been beneficial to university level hockey players because it refines better Low-density Lipoprotein (LDL) and High-density Lipoprotein (HDL)