Aims and Objectives: The aim of this in-vitro study is to evaluate the effect of different remineralizing agents on enamel erosion. Materials and Methods: A total of 100 extracted human maxillary and mandibular molar teeth were selected and cleaned with the ultrasonic scaler. The molars were sectioned using a disc cutting bur from the CEJ and the roots were removed from all the teeth. A 2 mm thickness of buccal surface was taken from all the teeth. The entire exposed surface was covered with clear nail polish to decrease the chance of accelerated demineralization caused by exposure of the dentin. Initial weights of all the teeth were taken. The teeth were stored in normal saline using different containers. The normal saline was decanted out of the containers just prior to commencement of the study. The teeth were then dipped in vinegar for 7 days for disinfection. Once the disinfection of the teeth was completed, the vinegar was decanted followed by its replacement with artificial saliva to all the containers to act as natural saliva. All the teeth were now randomly divided into five groups namely Group A, Group B, Group C, Group D and Group E. 20 teeth were allocated to each group and were kept in the container labelled as the remineralizing agent going to be used in it. Results: There is statistically significant difference present in remineralization in various groups. In this research after the treatment, Bifluoride 10 and Fluor Protector showed a significant difference between the experimental and control group. Remin Pro and Tooth Mousse Plus did not show a significant difference but the control group had a higher calcium concentration compared to the experimental group. Conclusion: This research elucidates the nuanced effects of remineralization agents on calcium dynamics within artificial saliva. The comparative analysis between experimental groups and the protective role of fluoride underscore the clinical relevance of these findings. While acknowledging the in-vitro limitations, the observed potential for remineralizing agents to contribute to enamel remineralization suggests promising avenues for future research and clinical applications.