Male infertility poses an emerging concern in public health circles. While surgical procedures and interventions are available, they come with hefty costs. Given their perceived safety and affordability, herbal remedies have gained popularity in addressing infertility. Hemidesmus indicus (HI), a traditional medicinal plant, is renowned for its purported efficacy in enhancing semen quality. Thus, this study aimed to evaluate the reproductive effects of Hemidesmus indicus (HI) extract on sperm abnormalities elicited by Monosodium Glutamate (MSG). Subfertile rats induced with Monosodium glutamate (MSG) were orally administered the ethanolic extract of Hemidesmus indicus (400mg/Kg body weight) for 30 days. The extract's impact on sperm count, motility, viability, morphology, testis weight, semen pH, was compared with control and MSG-treated groups. The results revealed that Hemidesmus indicus extract significantly boosted sperm count (p<0.001), and viability (p<0.001) motility (p<0.001), compared to the MSG group, which experienced notable declines in these parameters. Abnormal sperm morphology was significantly higher in the MSG group (p<0.001) but significantly reduced in the HIE group (p=0.007) and MSG+HIE group (p=0.001) compared to the MSG group. These findings indicate that Hemidesmus indicus extract mitigated the adverse effects of monosodium glutamate on sperm parameters, suggesting its potential in enhancing fertility and safeguarding the reproductive system against monosodium glutamate-induced damage.