Background: the volume of research that has studied the fatiguing effects on postural control is high, while studying the fatigue effect on balance and actual sport performance in football players has been of less attention. Objectives: to examine the impact of fatigue on core endurance and balance in addition to performance in adolescent football players. Subjects and methods: 60 male football players were randomized into one of two groups: one fatigue group, and the control group. Initially, all players were evaluated for core endurance, balance, as well as performance. After that, the fatigue group was given a fatigue protocol to follow. After that, all measurements were repeated. Study design: Randomized clinical study.Results:Within group comparison there was a significant difference between pre and post measurements for all variables of the fatigue group,while in the control group there was a non-significant difference between pre and post measurements of all variables.Between groupscomparison there was a non-significant difference of all outcome measures betweengroups in pre fatigue measurements,while post fatigue protocol there was a significant difference regarding balance and running sprint time and non-significant differenceregarding core endurance and Triple‐Hop Test. Conclusion: Fatiguehas a negative impact on adolescent football player'sbalanceand performance that could increase the risk of injury.