Caralluma fimbriata is a succulent plant highly esteemed in traditional medicine for its medicinal properties. Plant hormones, also known as phytohormones, are chemical compounds that regulate several aspects of plant growth and development. The current study demonstrates the establishment of callus culture and investigate the effect of JA (jasmonic acid) on callus in development of somatic embryos and indirect organogenesis from the internodal explants of C. fimbriata through micropropagation techniques. MS medium (full-strength) was supplied with different concentrations of auxins (2,4-D, IAA, IBA, NAA) and the optimal callus growth was observed in MS medium + 2,4-D (2.0 mg/l) + NAA (1.0 mg/l) with 100% of callus growth response. JA influenced in producing somatic embryos from the callus at almost all concentrations. Highest shoot induction response (80%) was observed on MS medium+ 0.5 mg/l BAP with highest mean frequency of 9.53 ± 1.20a, compared to Kn. Rooting was successfully accomplished on MS medium (half- strength) with supplementation of MS salts + 0.1 mg/l NAA. The survival rate of acclimatized plants was recorded at 80%.