Aims and objectives: The study aimed to assess the effectiveness of Mindfulness on depression, anxiety and stress among patients with type 2 diabetes. Approach and design: A quantitative approach and randomized control trial design was used. Samples and Sampling Criteria: The convenient sampling technique was used to find out eligible sample and then the lottery method of simple random sampling used to classify the samples into experimental & control group. The final samples participated in the intervention were 49 in the experimental group and 49 in the control group. Thus, finally the total samples for the study were 98. The tool was (DASS-42) Depression, Anxiety Stress Scale to assess depression, anxiety and stress in patients with type 2 diabetes. The investigator conducted a client centered and group centered Mindfulness sessions among experimental group. Results: Descriptive and inferential statistics were used to find out the effectiveness of Mindfulness among experimental and control group. The comparison of outcome of Mindfulness on depression, anxiety and stress among patients with type 2 diabetes in experimental group which was tested by using paired t test. The Comparison of outcome of Mindfulness on depression, anxiety and stress among patients with type 2 diabetes in experimental group and a wait-list controlled group which was tested by using unpaired t test. According to depression among patients with type 2 diabetes in experimental group mean score was 12.37±3.34 and in a wait-list control group was 15.18±2.61 with mean difference of 2.81 with calculated value t=4.645, df=96, p=0.001 was highly significant. With regard to anxiety among patients with type 2 diabetes in experimental group mean score was 13.47±2.99 and in a wait-list control group was 17.53±2.93 with mean difference of 4.06 with calculated value t=6.778, df=96, p=0.001 was highly significant. Regarding stress among patients with type 2 diabetes in experimental group mean score was 16.0±3.50 and in a wait-list control group was 18.98±2.43 with mean difference of 2.98 with calculated value t=4.891, df=96, p=0.001 was highly significant. Conclusion: Significant decrease in anxiety, depression, and stress in the experimental group in the post test was found. However, there were no significant changes in any of the aforesaid measures in the wait list control group.