Gluconeogenesis in the liver of streptozotocin (STZ)- prompted diabetic rats was the subject of this exploration, which investigated the effects of Achillea millefolium hydroalcoholic extract on the glucose creation process. In this examination, fifty male Sprague-Dawley rats were parted into seven gatherings and given various medicines over a time of 28 all out days. In contrast with both the gatherings that were treated with metformin and the benchmark group, the outcomes showed that the extract, particularly when regulated at a dose of 100 mg/kg/day, decidedly affected blood glucose levels, lipid levels, and liver catalyst results. Specifically, the extract brought about significant reductions in blood glucose levels as well as serum centralizations of absolute cholesterol, fatty substances (TG), and low-thickness lipoprotein (LDL)- cholesterol, while at the same time prompting an expansion in highthickness lipoprotein (HDL)- cholesterol levels. As per this information, there is plausible that Achillea millefolium extract could play a part in lessening the metabolic irregularities that are connected with diabetes mellitus.