The current research aims to design a rehabilitation program using Pilates exercises and identifying its effects on some bio-markers of kick-boxers with thigh adductor rupture. The researchers used the experimental approach (one-group design) with pre- and post-measurements. Research community included (24) kick-boxers of the Egyptian National Team with thigh adductor rupture. Pilot sample included (10) kick-boxers while the main sample included (14) players. Results indicated that:  The rehabilitation program using Pilates exercises improves biomarkers represented by muscle tear enzymes (transaminase enzymes – Creatine phosphokinase – acetylcholinesterase). This improvement results in a statistically significant reduction in muscle tears, as evidenced by the pre- and post-measurement differences in muscle tear indicators and the enzymes ALT, AST, and CPK.  Regular Pilates training leads to enhanced physical fitness, improved biological condition, and consequently, increased efficiency of the muscles working around the hip joint.