The emergence of antibiotic resistance in Staphylococcus aureus, particularly Vancomycin resistant Staphylococcus aureus (VRSA), has posed significant challenges in clinical treatment. This study investigates the potential synergistic effect of combining vancomycin with chrysin, a natural flavono id, to combat VRSA. Employing a multi faceted approach including disc diffusion assays, minimum inhibitory concentration (MIC) determination, and Scanning Electron Microscopy (SEM), we assessed the antimicrobial and antibiofilm efficacy of the vancomycin c hrysin combination. Our findings demonstrate enhanced antibacterial activity and significant disruption of biofilm structure, suggesting that chrysin augments the effectiveness of vancomycin against VRSA. These results offer promising insights into alternative therapeutic strategies for treating antibiotic resistant infections.